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Conservatoire botanique national des Pyrénées et de Midi-Pyrénées

Assessment of genetic relationships among sexual and asexual forms of Allium cepa using morphological traits and RAPD markers / Maud Le Thierry D'Ennequin in Heredity, 78 (1997) 

Public Citation [article]  inHeredity > 78 (1997) . - 403-409 Titre : Assessment of genetic relationships among sexual and asexual forms of Allium cepa using morphological traits and RAPD markers Type de document : Numérique Auteurs : Maud Le Thierry D'Ennequin ; Olivier Panaud ; Thierry Robert ; Agnès Ricroch Année de publication : 1997 Article en page(s) : 403-409 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Distribution de la diversité génétique [CBNPMP-Thématique] Système de reproduction [CBNPMP-Thématique] Variabilité, analyse de l'ADN, polymorphisme Mots-clés : Allium cepa L. Résumé : The species Allium cepa includes two major crops on the basis of morphological traits and typical reproduction mode: sexually reproduced biennial onions and vegetatively propagated perennial shallots which rarely flower. In addition, the seed-propagated shallot, a recently released variety with intermediate phenotype for life history, has been described and used by breeders. A joint analysis using molecular markers (random amplified polymorphic DNA, RAPD) and morphological characters was undertaken for the assessment of genetic diversity and crop classification among Allium cepa genotypes. A morphological study describing growth and development was carried out on a nested sampling (34 accessions) from two geographical origins (European or tropical) and of different plant status (improved varieties or local ecotypes). Four primers generated 24 reproducibly scorable DNA bands which gave individual fingerprinting for representative accessions of both onions and shallots. Our results indicate that the seed-propagated shallot is more closely related to onions than to vegetatively propagated shallots and, moreover, reveal a geographical structure of genetic diversity. The evolutionary significance of these data is discussed. Identifiant : DOI : 10.1038/hdy.1997.63 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=156308 Le Thierry D'Ennequin, M., Panaud, O., Robert, T., Ricroch, A., 1997 . Assessment of genetic relationships among sexual and asexual forms of Allium cepa using morphological traits and RAPD markers. Heredity, 78: 403-409.

Documents numériques

Article (1997) Adobe Acrobat PDF

Whole plastid genome-based phylogenomics supports an inner placement of the O. insectifera group rather than a basal position in the rapidly diversifying Ophrys genus (Orchidaceae) / Joris A. M. Bertrand in Botany letters, 168 (3) (2021)

Public Citation [article]  inBotany letters > 168 (3) (2021) . - 452-457 Titre : Whole plastid genome-based phylogenomics supports an inner placement of the O. insectifera group rather than a basal position in the rapidly diversifying Ophrys genus (Orchidaceae) Type de document : Imprimé Auteurs : Joris A. M. Bertrand ; Anaïs Gibert ; Christel LLauro ; Olivier Panaud Année de publication : 2021 Article en page(s) : 452-457 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Génétique et écologie (dynamique, démographique, sélection) Mots-clés : ophrys insectifera Résumé : Some lineages of the orchid genus Ophrys exhibit among the highest diversification rates reported so far. As a consequence of a such intense and rapid evolution, the systematics and taxonomy of this genus remain unclear. A hybrid assembly approach based-on long- and short-read genomic data allowed us to outperform classical methods to successfully assemble whole plastid genomes for two new species of Ophrys: O. aymoninii and O. lutea. Along with three other previously Ophrys plastid genome sequences, we then reconstructed the first whole plastome-based molecular phylogeny including representatives of the three mains recognized Ophrys lineages. Our results support the placement of the O. insectifera clade as sister group of “non-basal Ophrys” rather than a basal position. Our findings corroborate recent results obtained from genomic data (RAD-seq and transcriptomes) but contrast with previous ones. These results therefore confirm that molecular phylogenetic hypotheses based on a limited number of loci (e.g. nrITS, matK, rbcL) may have provided a biased picture of phylogenetic relationships within Ophrys and possibly other plant taxa. Identifiant : DOI : 10.1080/23818107.2021.1893216 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=146886 Bertrand, J.A.M., Gibert, A., LLauro, C., Panaud, O., 2021 . Whole plastid genome-based phylogenomics supports an inner placement of the O. insectifera group rather than a basal position in the rapidly diversifying Ophrys genus (Orchidaceae). Botany letters, 168(3): 452-457.

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Code-barres	Cote	Support	Localisation	Section	Disponibilité
R20637	P-1824	Revue	Centre de documentation	Revues	Consultable