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Auteur Pierre Taberlet |
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Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers / Nathalie Escavarage (1998)
Titre : Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers Type de document : Tiré à part de revue Auteurs : Nathalie Escavarage ; S. Questiau ; Université Joseph Fourier (Grenoble) ; André Pornon (1943-) ; Université de Rennes 1 ; Bernard Doche (1950-) ; Pierre Taberlet Mention d'édition : 1998 Année de publication : 1998 Importance : 975-982 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Génétique et écologie (dynamique, démographique, sélection) Mots-clés : Rhododendron ferrugineum L. Résumé : In the European Alps, Rhododendron ferrugineum can constitute dense populations with almost 100% of cover. The developmental pattern by layering and the resulting complexity of population structure make it difficult to identify distinct clones even by excavation. Therefore genotypic structure of a R. ferrugineum population, in the French Alps, was inferred from AFLP markers. In a first step, we analysed 400 samples using AFLP profiles generated by one selective primer pair. Seventeen bands out of 25 were polymorphic (68%). We identified a total of 32 multilocus genotypes. In a second step, the 32 genotypes were verified by applying two additional primer pairs to the two most distant samples from each genotype. The mean similarity (proportion of band sharing) between pairs of clones was 0.85 (range from 0.52 to 0.94). The spatial distribution of clones showed that vegetative spreading mainly occurred down a slope. Based on an annual shoot mean growth of 2.6 cm/year and the size of the widest clone, we estimated the age of the oldest individual to be at least 300 years. A single genotype can occupy a large surface and sometimes form a dense patch, suggesting that this species adopts a phalanx growth form with limited intermingling of some genets. Lien pérenne : DOI : 10.1046/j.1365-294x.1998.00415.x Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=129968 Escavarage, Nathalie, Questiau, S., Université Joseph Fourier (Grenoble), Pornon, André (1943-), Université de Rennes 1, Doche, Bernard (1950-), Taberlet, Pierre 1998 Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers. Molecular ecology, 7 : 975-982.Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 7180 Escavarage N. Tiré à part Centre de documentation Tirés à part Consultable Dynamics of genotypic structure in clonal Rhododendron ferrugineum (Ericaceae) populations / André Pornon (2000)
Titre : Dynamics of genotypic structure in clonal Rhododendron ferrugineum (Ericaceae) populations Type de document : Tiré à part de revue Auteurs : André Pornon (1943-) ; Nathalie Escaravage ; P. Thomas ; Pierre Taberlet Année de publication : 2000 Importance : 1099-1111 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Forme de vie et structure de la population végétale
[CBNPMP-Thématique] Génétique et écologie (dynamique, démographique, sélection)
[CBNPMP-Thématique] Système de reproduction
[CBNPMP-Thématique] Variabilité, analyse de l'ADN, polymorphismeMots-clés : Rhododendron ferrugineum L. Résumé : Two populations of Rhododendron ferrugineum growing at subalpine level in the Pyrenees (France) were studied in two sites (Bethmale and Mourtis). Identification and delimitation of genets were inferred from amplified fragment length polymorphism (AFLP) markers, along a closure gradient (from meadow to more closed heath) in each site. Surface and age of genets, genotypic diversity (Simpson's index D), 'proportion distinguishable' genotypes and genetic relationships between genets were then estimated. Amplification of the 312 DNA samples with three selective primer pairs gave a mean of 98 detectable peaks (i.e. bands) per sample, with size ranging from 60 to 300 bp. In total 60% (Bethmale) and 70% (Mourtis) of the peaks were polymorphic, and a total of 31 and 23 multilocus genotypes were identified, in Bethmale and Mourtis, respectively. We inferred that pioneer genotypes began arriving 110 years ago mainly over a 40-year period in the Mourtis meadow and began about 130 years ago over a 100-year period in the Bethmale meadow After this pioneer stage, populations extended vegetatively. Two different patterns of genotypic dynamics can be identified. At Bethmale, population closure could have led to a dramatic loss of genets and to the selection of highly genetically related genotypes. In contrast, at Mourtis, genotypic diversity and genet density did not change fundamentally along the closure gradient. However the range of genetic diversity diminished from the open to the closed situation, suggesting that thinning could have occurred in the past. Lien pérenne : DOI : 10.1046/j.1365-294x.2000.00976.x Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=130586 Pornon, André (1943-), Escaravage, Nathalie, Thomas, P., Taberlet, Pierre 2000 Dynamics of genotypic structure in clonal Rhododendron ferrugineum (Ericaceae) populations. Molecular ecology, 9 : 1099-1111.Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 9392 Pornon A. Tiré à part Centre de documentation Tirés à part Consultable Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers / Myriam Gaudeul in Molecular ecology, 9 (2000)
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Titre : Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers Type de document : Imprimé Auteurs : Myriam Gaudeul (1976-) ; Pierre Taberlet ; Irène Till-Bottraud Année de publication : 2000 Article en page(s) : 1625-1637 Langues : Anglais (eng) Mots-clés : Eryngium alpinum L., 1753 Résumé : Eryngium alpinum L. is an endangered species found across the European Alps. In order to obtain base-line data for the conservation of this species, we investigated levels of genetic diversity within and among 14 populations from the French Alps. We used the amplified fragment length polymorphism (AFLP) technique with three primer pairs and scored a total of 62 unambiguous, polymorphic markers in 327 individuals. Because AFLP markers are dominant, within-population genetic structure (e.g. FIS) could not be assessed. Analyses based either on the assumption of random-mating or on complete selfing lead to very similar results. Diversity levels within populations were relatively high (mean Nei's expected heterozygosity = 0.198; mean Shannon index = 0.283), and a positive correlation was detected between both genetic diversity measurements and population size (Spearman rank correlation: P = 0. 005 and P = 0.002, respectively). Moreover, FST values and exact tests of differentiation revealed high differentiation among populations (mean pairwise FST = 0.40), which appeared to be independent of geographical distance (nonsignificant Mantel test). Founder events during postglacial colonizations and/or bottlenecks are proposed to explain this high but random genetic differentiation. By contrast, we detected a pattern of isolation by distance within populations and valleys. Predominant local gene flow by pollen or seed is probably responsible for this pattern. Concerning the management of E. alpinum, the high genetic differentiation leads us to recommend the conservation of a maximum number of populations. This study demonstrates that AFLP markers enable a quick and reliable assessment of intraspecific genetic variability in conservation genetics. Lien pérenne : DOI : 10.1046/j.1365-294x.2000.01063.x Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=148157
in Molecular ecology > 9 (2000) . - 1625-1637Gaudeul, Myriam (1976-), Taberlet, Pierre, Till-Bottraud, Irène 2000 Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers. Molecular ecology, 9: 1625-1637.Genetic introgression as a potential to widen a species' niche : insights from alpine Carex curvula / Philippe Choler (2004)
est un tiré à part de 101 (1) - 01/2004 (Proceedings of the National Academy of Sciences of the United States of America)
Titre : Genetic introgression as a potential to widen a species' niche : insights from alpine Carex curvula Type de document : Tiré à part de revue Auteurs : Philippe Choler ; B Erschbamer ; Andreas Tribsch ; Ludovic Gielly ; Pierre Taberlet Année de publication : 2004 Importance : 171-176 Langues : Anglais (eng) Lien pérenne : DOI : 10.1073/pnas.2237235100 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=131256 Choler, Philippe, Erschbamer, B, Tribsch, Andreas, Gielly, Ludovic, Taberlet, Pierre 2004 Genetic introgression as a potential to widen a species' niche : insights from alpine Carex curvula. Proceedings of the National Academy of Sciences of the United States of America, 101(1) : 171-176.Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 13854 C Tiré à part Centre de documentation Tirés à part Consultable Documents numériques
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Article (2004)URL Long livestock farming history and human landscape shaping revealed by lake sediment DNA / Charline Giguet-Covex in Nature communications, 5 (2014)
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Titre : Long livestock farming history and human landscape shaping revealed by lake sediment DNA Type de document : Imprimé Auteurs : Charline Giguet-Covex ; Johan Pansu ; Fabien Arnaud ; Pierre-Jérôme Rey ; Christophe Griggo ; Ludovic Gielly ; Isabelle Domaison ; Eric Coissac ; Fernand David ; Philippe Choler ; Jérôme Poulenard ; Pierre Taberlet Année de publication : 2014 Article en page(s) : 3211 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Enjeux de conservation des lacs d'altitude Résumé : The reconstruction of human-driven, Earth-shaping dynamics is important for understanding past human/environment interactions and for helping human societies that currently face global changes. However, it is often challenging to distinguish the effects of the climate from human activities on environmental changes. Here we evaluate an approach based on DNA metabarcoding used on lake sediments to provide the first high-resolution reconstruction of plant cover and livestock farming history since the Neolithic Period. By comparing these data with a previous reconstruction of erosive event frequency, we show that the most intense erosion period was caused by deforestation and overgrazing by sheep and cowherds during the Late Iron Age and Roman Period. Tracking plants and domestic mammals using lake sediment DNA (lake sedDNA) is a new, promising method for tracing past human practices, and it provides a new outlook of the effects of anthropogenic factors on landscape-scale changes. Lien pérenne : DOI : 10.1038/ncomms4211 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=150974
in Nature communications > 5 (2014) . - 3211Giguet-Covex, Charline, Pansu, Johan, Arnaud, Fabien, Rey, Pierre-Jérôme, Griggo, Christophe, Gielly, Ludovic, Domaison, Isabelle, Coissac, Eric, David, Fernand, Choler, Philippe, Poulenard, Jérôme, Taberlet, Pierre 2014 Long livestock farming history and human landscape shaping revealed by lake sediment DNA. Nature communications, 5: 3211.Documents numériques
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Article (2014)URL