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Comparative ITS and AFLP analysis of diploid cardamine (brassicaceae) taxa from closely related polyploid complexes / Karol Marhold in Annals of Botany, 93 (2004) 

Public Citation [article]  inAnnals of Botany > 93 (2004) . - 507-520 Titre : Comparative ITS and AFLP analysis of diploid cardamine (brassicaceae) taxa from closely related polyploid complexes Type de document : Imprimé Auteurs : Karol Marhold ; Judita Lihová ; Marián Perny ; Walter Bleeker Année de publication : 2004 Article en page(s) : 507-520 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Phylogénie (liens existant entre espèces apparentées) Mots-clés : Cardamine pratensis L.  Cardamine raphanifolia  Cardamine amara L. Résumé : Background and aims: Diploid representatives from the related polyploid complexes of Cardamine amara, C. pratensis and C. raphanifolia (Brassicaceae), were studied to elucidate phylogenetic relationships among the complexes and among the individual taxa included. Methods: Two independent molecular data sets were used: nucleotide sequences from the internal transcribed spacers (ITS) of nrDNA, and amplified fragment length polymorphism (AFLP) markers. Seventeen diploid taxa from the studied groups were sampled. Key results: Both ITS and AFLP analyses provided congruent results in inferred relationships, and revealed two main lineages. While the C. amara group, consisting of C. wiedemanniana and four subspecies of C. amara, was resolved as a well-supported monophyletic group, taxa from the C. pratensis and C. tenera groups (the latter representing diploid taxa of the complex of C. raphanifolia) all appeared together in a single clade/cluster with no support for the recognition of either of the groups. Intra-individual polymorphisms and patterns of nucleotide variation in the ITS region in C. uliginosa and C. tenera, together with the distribution of AFLP bands, indicate ancient hybridization and introgression among these Caucasian diploids. Conclusions: The lack of supported hierarchical structure suggests that extensive reticulate evolution between these groups, even at the diploid level, has occurred (although an alternative explanation, namely ancestral polymorphism in ITS data, cannot be completely excluded). Several implications for the investigation of the polyploid complexes of concern are drawn. When tracing origins of polyploid taxa, a much more complex scenario should be expected, taking into account all relatives as potential parents, irrespective of the group in which they are classified. Lien pérenne : DOI : 10.1093/aob/mch073 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=149111 Marhold, Karol, Lihová, Judita, Perny, Marián, Bleeker, Walter 2004 Comparative ITS and AFLP analysis of diploid cardamine (brassicaceae) taxa from closely related polyploid complexes. Annals of Botany, 93: 507-520.

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Consultable Article (2004) URL

Natural hybridization in Cardamine (Brassicaceae) in the Pyrenees: evidence from morphological and molecular data / Karol Marhold in Botanical journal of the Linnean Society, 139 (2002) 

Public Citation [article]  inBotanical journal of the Linnean Society > 139 (2002) . - 275-294 Titre : Natural hybridization in Cardamine (Brassicaceae) in the Pyrenees: evidence from morphological and molecular data Type de document : Imprimé Auteurs : Karol Marhold ; Judita Lihová ; Marián Perny ; Rudi Grupe Année de publication : 2002 Article en page(s) : 275-294 Langues : Français (fre) Catégories : [CBNPMP-Thématique] Génétique et écologie (dynamique, démographique, sélection) Mots-clés : Cardamine Résumé : While researching Cardamine (Brassicaceae) in the Pyrenees, putative hybrid plants were found at two natural sites. Pollen grain viability, AFLP, and multivariate morphometric analyses were performed in order to assess the plants' presumed hybrid origin, establishing that natural hybridization between the diploids C. crassifolia and C. amara ssp. pyrenaea had occurred. A new diploid nothospecies, C.×enriquei (2n = 2x = 16), is described. Examination of 18 morphological characters showed the intermediacy of the hybrid between the parental taxa in most characters. AFLP analyses of C. amara ssp. amara, ssp. austriaca, ssp. olotensis and C. raphanifolia, demonstrated the close position of the hybrid to C. crassifolia, and revealed that the highest number of markers were shared with the parents. Polymorphism found in the AFLP pattern of the hybrid suggested recurrent origin, segregation and/or backcrosses, although assessment of pollen viability indicated high male sterility. The hybridization event reported here represents the second documented case between the C. pratensis group and C. amara. An account of the nomenclature of C. crassifolia is also presented, including lectotypification of relevant names. Lien pérenne : DOI : 10.1046/j.1095-8339.2002.00066.x Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=149119 Marhold, Karol, Lihová, Judita, Perny, Marián, Grupe, Rudi 2002 Natural hybridization in Cardamine (Brassicaceae) in the Pyrenees: evidence from morphological and molecular data. Botanical journal of the Linnean Society, 139: 275-294.

Documents numériques

Consultable Article (2002) URL