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eDNA Increases the Detectability of Ranavirus Infection in an Alpine Amphibian Population / Claude Miaud in Viruses, 11(6) (526) (2019) 

Public Citation [article]  inViruses > 11(6) (526) (2019) Titre : eDNA Increases the Detectability of Ranavirus Infection in an Alpine Amphibian Population Type de document : Imprimé Auteurs : Claude Miaud ; Véronique Arnal ; Marie Poulain ; Alice Valentini ; Tony Dejean Année de publication : 2019 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Lac d'altitude Résumé : The early detection and identification of pathogenic microorganisms is essential in order to deploy appropriate mitigation measures. Viruses in the Iridoviridae family, such as those in the Ranavirus genus, can infect amphibian species without resulting in mortality or clinical signs, and they can also infect other hosts than amphibian species. Diagnostic techniques allowing the detection of the pathogen outside the period of host die-off would thus be of particular use. In this study, we tested a method using environmental DNA (eDNA) on a population of common frogs (Rana temporaria) known to be affected by a Ranavirus in the southern Alps in France. In six sampling sessions between June and September (the species’ activity period), we collected tissue samples from dead and live frogs (adults and tadpoles), as well as insects (aquatic and terrestrial), sediment, and water. At the beginning of the breeding season in June, one adult was found dead; at the end of July, a mass mortality of tadpoles was observed. The viral DNA was detected in both adults and tadpoles (dead or alive) and in water samples, but it was not detected in insects or sediment. In live frog specimens, the virus was detected from June to September and in water samples from August to September. Dead tadpoles that tested positive for Ranavirus were observed only on one date (at the end of July). Our results indicate that eDNA can be an effective alternative to tissue/specimen sampling and can detect Ranavirus presence outside die-offs. Another advantage is that the collection of water samples can be performed by most field technicians. This study confirms that the use of eDNA can increase the performance and accuracy of wildlife health status monitoring and thus contribute to more effective surveillance programs. Lien pérenne : DOI : 10.3390/v11060526 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=152375 Miaud, Claude, Arnal, Véronique, Poulain, Marie, Valentini, Alice, Dejean, Tony 2019 eDNA Increases the Detectability of Ranavirus Infection in an Alpine Amphibian Population. Viruses, 11(6)(526).

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Consultable Article (2019) URL

Recent Asian origin of chytrid fungi causing global amphibian declines / Simon O’Hanlon in Science, 360 (2018) 

Public Citation [article]  inScience > 360 (2018) . - 621-627 Titre : Recent Asian origin of chytrid fungi causing global amphibian declines Type de document : Imprimé Auteurs : Simon O’Hanlon ; Adrien Rieux ; Rhys A. Farrer ; Gonçalo M. Rosa ; Bruce Waldman ; Arnaud Bataille ; Tiffany A. Kosch ; Kris A. Murray ; Balázs Brankovics ; Matteo Fumagalli ; Michael D. Martin ; Nathan Wales ; Mario Alvarado-Rybak ; Kieran A. Bates ; Lee Berger ; Suzanne Böll ; Lola Brookes ; Frances C. Clare ; Elodie A. Courtois ; Andrew A. Cunningham ; Thomas M. Doherty-Bone ; Pria Gosh ; David J. Gower ; William E. Hintz ; Jacob Höglund ; Thomas S. Jenkinson ; Chun-fu Lin ; Anssi Laurila ; Adeline Loyau ; An Martel ; Sara Meurling ; Claude Miaud ; Pete Minting ; Frank Pasmans ; Dirk S. Schmeller ; Benedikt Schmidt ; Jennifer M. G. Shelton ; Lee F. Skerratt ; Freya Smith ; Claudio Soto-Azat ; Matteo Spagnoletti ; Giulia Tessa ; Luís Felipe Toledo ; Andrés Valenzuella-Sánchez ; Ruhan Verster ; Judith Vörös ; Rebecca J. Webb ; Claudia Wierzbicki ; Emma Wombwell ; Kelly R. Zamudio ; David M. Aanensen ; Timothy Y. James ; M. Thomas P. Gilbert ; Ché Weldon ; Jaime Bosch ; François Balloux ; Trenton W. J. Garner ; Matthew C. Fisher Année de publication : 2018 Article en page(s) : 621-627 Langues : Anglais (eng) Catégories : [CBNPMP-Thématique] Lac d'altitude Résumé : Globalized infectious diseases are causing species declines worldwide, but their source often remains elusive. We used whole-genome sequencing to solve the spatiotemporal origins of the most devastating panzootic to date, caused by the fungus Batrachochytrium dendrobatidis, a proximate driver of global amphibian declines. We traced the source of B. dendrobatidis to the Korean peninsula, where one lineage, BdASIA-1, exhibits the genetic hallmarks of an ancestral population that seeded the panzootic. We date the emergence of this pathogen to the early 20th century, coinciding with the global expansion of commercial trade in amphibians, and we show that intercontinental transmission is ongoing. Our findings point to East Asia as a geographic hotspot for B. dendrobatidis biodiversity and the original source of these lineages that now parasitize amphibians worldwide. Lien pérenne : DOI : 10.1126/science.aar1965 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=152317 O’Hanlon, Simon, Rieux, Adrien, Farrer, Rhys A., Rosa, Gonçalo M., Waldman, Bruce, Bataille, Arnaud, Kosch, Tiffany A., Murray, Kris A., Brankovics, Balázs, Fumagalli, Matteo, Martin, Michael D., Wales, Nathan, Alvarado-Rybak, Mario, Bates, Kieran A., Berger, Lee, Böll, Suzanne, Brookes, Lola, Clare, Frances C., Courtois, Elodie A., Cunningham, Andrew A., Doherty-Bone, Thomas M., Gosh, Pria, Gower, David J., Hintz, William E., Höglund, Jacob, Jenkinson, Thomas S., Lin, Chun-fu, Laurila, Anssi, Loyau, Adeline, Martel, An, Meurling, Sara, Miaud, Claude, Minting, Pete, Pasmans, Frank, Schmeller, Dirk S., Schmidt, Benedikt, Shelton, Jennifer M. G., Skerratt, Lee F., Smith, Freya, Soto-Azat, Claudio, Spagnoletti, Matteo, Tessa, Giulia, Toledo, Luís Felipe, Valenzuella-Sánchez, Andrés, Verster, Ruhan, Vörös, Judith, Webb, Rebecca J., Wierzbicki, Claudia, Wombwell, Emma, Zamudio, Kelly R., Aanensen, David M., James, Timothy Y., Gilbert, M. Thomas P., Weldon, Ché, Bosch, Jaime, Balloux, François, Garner, Trenton W. J., Fisher, Matthew C. 2018 Recent Asian origin of chytrid fungi causing global amphibian declines. Science, 360: 621-627.

Documents numériques

Consultable Article (2018) URL