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Conquérir l'espace : les stratégies de reproduction des plantes alpines en haute altitude / Serge Aubert in Hommes & Plantes, 57 (2006)

Public Citation [article]  inHommes & Plantes > 57 (2006) . - 34-39 Titre : Conquérir l'espace : les stratégies de reproduction des plantes alpines en haute altitude Type de document : Imprimé Auteurs : Serge Aubert (1966-2015) ; Philippe Cholet ; Rolland Douzet ; Irène Till-Bottraud Année de publication : 2006 Article en page(s) : 34-39 Langues : Français (fre) Catégories : [Thématique] Système de reproduction [Thématique] Rôle de l'altitude en physiologie végétale Aubert, S., Cholet, P., Douzet, R., Till-Bottraud, I., 2006 - Conquérir l'espace : les stratégies de reproduction des plantes alpines en haute altitude ; Hommes & Plantes, 57 : 34-39.

Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers / Myriam Gaudeul in Heredity, 92 (2004) 

Public Citation [article]  inHeredity > 92 (2004) . - 508–518 Titre : Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers Type de document : Imprimé Auteurs : Myriam Gaudeul (1976-) ; Irène Till-Bottraud ; Stéphanie Manel Année de publication : 2004 Article en page(s) : 508–518 Langues : Anglais (eng) Mots-clés : Eryngium alpinum L., 1771  Eryngium alpinum L., 1753 Résumé : Genetic diversity and structure of 12 populations of Eryngium alpinum L. were investigated using 63 dominant amplified fragment length polymorphism (AFLP) and seven codominant microsatellite (48 alleles) markers. Within-population diversity estimates obtained with both markers were not correlated, but the microsatellite-based fixation index Fis was correlated with both AFLP diversity indices (number of polymorphic bands and Nei's expected heterozygosity). Only AFLP diversity indices increased with the size of populations, although they did not significantly differ among them (Kruskall–Wallis test). The discrepancy between AFLPs and microsatellites may be explained by a better coverage of the genome with numerous AFLPs, the higher mutation rates of microsatellites or the absence of significant difference among within-population diversity estimates. Genetic differentiation was higher with AFLPs (θ=0.40) than with microsatellites (θ=0.23), probably due to the higher polymorphism of microsatellites. Thus, we considered global qualitative patterns rather than absolute estimates to compare the performance of both types of markers. On a large geographic scale, the Mantel test and multivariate analysis showed that genetic patterns were more congruent with the spatial arrangement of populations when inferred from microsatellites than from AFLPs, suggesting higher homoplasy of AFLP markers. On a small spatial scale, AFLPs managed to discriminate individuals from neighboring populations whereas microsatellites did not (multivariate analysis), and the percentage of individuals correctly assigned to their population of origin was higher with AFLPs than with microsatellites. However, dominant AFLPs cannot be used to study heterozygosity-related topics. Thus, distinct molecular markers should be used depending on the biological question and the geographical scale investigated. Identifiant pérenne : DOI : 10.1038/sj.hdy.6800443 Gaudeul, M., Till-Bottraud, I., Manel, S., 2004 - Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers ; Heredity, 92 : 508–518.

Documents numériques

Consultable Article (2004) Adobe Acrobat PDF

Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers / Myriam Gaudeul in Molecular Ecology, 9 (2000)

Public Citation [article]  inMolecular Ecology > 9 (2000) . - 1625-1637 Titre : Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers Type de document : Imprimé Auteurs : Myriam Gaudeul (1976-) ; Pierre Taberlet ; Irène Till-Bottraud Année de publication : 2000 Article en page(s) : 1625-1637 Langues : Anglais (eng) Mots-clés : Eryngium alpinum L., 1753 Résumé : Eryngium alpinum L. is an endangered species found across the European Alps. In order to obtain base-line data for the conservation of this species, we investigated levels of genetic diversity within and among 14 populations from the French Alps. We used the amplified fragment length polymorphism (AFLP) technique with three primer pairs and scored a total of 62 unambiguous, polymorphic markers in 327 individuals. Because AFLP markers are dominant, within-population genetic structure (e.g. FIS) could not be assessed. Analyses based either on the assumption of random-mating or on complete selfing lead to very similar results. Diversity levels within populations were relatively high (mean Nei's expected heterozygosity = 0.198; mean Shannon index = 0.283), and a positive correlation was detected between both genetic diversity measurements and population size (Spearman rank correlation: P = 0. 005 and P = 0.002, respectively). Moreover, FST values and exact tests of differentiation revealed high differentiation among populations (mean pairwise FST = 0.40), which appeared to be independent of geographical distance (nonsignificant Mantel test). Founder events during postglacial colonizations and/or bottlenecks are proposed to explain this high but random genetic differentiation. By contrast, we detected a pattern of isolation by distance within populations and valleys. Predominant local gene flow by pollen or seed is probably responsible for this pattern. Concerning the management of E. alpinum, the high genetic differentiation leads us to recommend the conservation of a maximum number of populations. This study demonstrates that AFLP markers enable a quick and reliable assessment of intraspecific genetic variability in conservation genetics. Identifiant pérenne : DOI : 10.1046/j.1365-294x.2000.01063.x Gaudeul, M., Taberlet, P., Till-Bottraud, I., 2000 - Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers ; Molecular Ecology, 9 : 1625-1637.

Intraspecific genetic diversity in alpine plants / Irène Till-Bottraud (2002)

Public Citation in Mountain biodiversity : a global assesment / Christian Körner (2002) Titre : Intraspecific genetic diversity in alpine plants Type de document : Extrait d'ouvrage Auteurs : Irène Till-Bottraud ; Myriam Gaudeul (1976-) Année de publication : 2002 Importance : p. 23-34 Langues : Anglais (eng) Catégories : [Thématique] Biodiversité Till-Bottraud, I., Gaudeul, M., 2002 - Intraspecific genetic diversity in alpine plants ; in : Mountain biodiversity : a global assesment, 2002, p. 23-34.

Menaces sur l'or bleu : étude de la diversité génétique des lavandes fines sauvages et cultivées / Cyrille Conord (2012)

Public Citation in Les actes des 2es rencontres végétales du Massif central / Conservatoire botanique national du Massif central (2012)   Titre : Menaces sur l'or bleu : étude de la diversité génétique des lavandes fines sauvages et cultivées Type de document : Extrait d'ouvrage Auteurs : Cyrille Conord ; Yolande Despinasse ; Sandrine Moja ; Delphine Rioux ; Irène Till-Bottraud ; F Jullien ; Florence Nicolè Année de publication : 2012 Importance : p. 147-154 Langues : Français (fre) Catégories : [Thématique] Cueillette Mots-clés : Lavandula angustifolia Mill., 1768 Conord, C., Despinasse, Y., Moja, S., Rioux, D., Till-Bottraud, I., Jullien, F., Nicolè, F., 2012 - Menaces sur l'or bleu : étude de la diversité génétique des lavandes fines sauvages et cultivées ; in : Les actes des 2es rencontres végétales du Massif central, 2012, p. 147-154.

Méthodologie de suivi des espèces végétales rares mise en place par un réseau d'acteurs de la conservation / Véronique Bonnet in Acta botanica gallica, 162 (1) (01/2015) 

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Organisation du pool génétique de Setaria italica (L.) P. Beauv. et exploitation des ressources génétiques d'espèces spontanées / R. Zangré (1992)

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Reproductive ecology of the endangered alpine species eryngium alpinum l. (apiaceae) : phenology, gene dispersal and reproductive success / Myriam Gaudeul in Annals of Botany, 93 (2004) 

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Variation of reproductive traits in Rhododendron ferrugineum L. (Ericaceae) populations along a successional gradient / André Pornon (1997)

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