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Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers / Myriam Gaudeul in Heredity, 92 (2004)
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Titre : Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers Type de document : Imprimé Auteurs : Myriam Gaudeul (1976-) ; Irène Till-Bottraud ; Stéphanie Manel Année de publication : 2004 Article en page(s) : 508–518 Langues : Anglais (eng) Mots-clés : Eryngium alpinum L., 1771 Eryngium alpinum L., 1753 Résumé : Genetic diversity and structure of 12 populations of Eryngium alpinum L. were investigated using 63 dominant amplified fragment length polymorphism (AFLP) and seven codominant microsatellite (48 alleles) markers. Within-population diversity estimates obtained with both markers were not correlated, but the microsatellite-based fixation index Fis was correlated with both AFLP diversity indices (number of polymorphic bands and Nei's expected heterozygosity). Only AFLP diversity indices increased with the size of populations, although they did not significantly differ among them (Kruskall–Wallis test). The discrepancy between AFLPs and microsatellites may be explained by a better coverage of the genome with numerous AFLPs, the higher mutation rates of microsatellites or the absence of significant difference among within-population diversity estimates. Genetic differentiation was higher with AFLPs (θ=0.40) than with microsatellites (θ=0.23), probably due to the higher polymorphism of microsatellites. Thus, we considered global qualitative patterns rather than absolute estimates to compare the performance of both types of markers. On a large geographic scale, the Mantel test and multivariate analysis showed that genetic patterns were more congruent with the spatial arrangement of populations when inferred from microsatellites than from AFLPs, suggesting higher homoplasy of AFLP markers. On a small spatial scale, AFLPs managed to discriminate individuals from neighboring populations whereas microsatellites did not (multivariate analysis), and the percentage of individuals correctly assigned to their population of origin was higher with AFLPs than with microsatellites. However, dominant AFLPs cannot be used to study heterozygosity-related topics. Thus, distinct molecular markers should be used depending on the biological question and the geographical scale investigated. Lien pérenne : DOI : 10.1038/sj.hdy.6800443 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=140706
in Heredity > 92 (2004) . - 508–518Gaudeul, M., Till-Bottraud, I., Manel, S. 2004. Genetic diversity and differentiation in Eryngium alpinum L. (Apiaceae): comparison of AFLP and microsatellite markers. Heredity, 92: 508–518.Documents numériques
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Article (2004)URLGenetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers / Myriam Gaudeul in Molecular ecology, 9 (2000)
[article]
Titre : Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers Type de document : Imprimé Auteurs : Myriam Gaudeul (1976-) ; Pierre Taberlet ; Irène Till-Bottraud Année de publication : 2000 Article en page(s) : 1625-1637 Langues : Anglais (eng) Mots-clés : Eryngium alpinum L., 1753 Résumé : Eryngium alpinum L. is an endangered species found across the European Alps. In order to obtain base-line data for the conservation of this species, we investigated levels of genetic diversity within and among 14 populations from the French Alps. We used the amplified fragment length polymorphism (AFLP) technique with three primer pairs and scored a total of 62 unambiguous, polymorphic markers in 327 individuals. Because AFLP markers are dominant, within-population genetic structure (e.g. FIS) could not be assessed. Analyses based either on the assumption of random-mating or on complete selfing lead to very similar results. Diversity levels within populations were relatively high (mean Nei's expected heterozygosity = 0.198; mean Shannon index = 0.283), and a positive correlation was detected between both genetic diversity measurements and population size (Spearman rank correlation: P = 0. 005 and P = 0.002, respectively). Moreover, FST values and exact tests of differentiation revealed high differentiation among populations (mean pairwise FST = 0.40), which appeared to be independent of geographical distance (nonsignificant Mantel test). Founder events during postglacial colonizations and/or bottlenecks are proposed to explain this high but random genetic differentiation. By contrast, we detected a pattern of isolation by distance within populations and valleys. Predominant local gene flow by pollen or seed is probably responsible for this pattern. Concerning the management of E. alpinum, the high genetic differentiation leads us to recommend the conservation of a maximum number of populations. This study demonstrates that AFLP markers enable a quick and reliable assessment of intraspecific genetic variability in conservation genetics. Lien pérenne : DOI : 10.1046/j.1365-294x.2000.01063.x Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=148157
in Molecular ecology > 9 (2000) . - 1625-1637Gaudeul, M., Taberlet, P., Till-Bottraud, I. 2000. Genetic diversity in an endangered alpine plant, Eryngium alpinum L. (Apiaceae), inferred from amplified fragment length polymorphism markers. Molecular ecology, 9: 1625-1637.How much do we know and how much do we care about genetic diversity of threatened plants ? A case study from the French flora / Juliette Ducrettet in Botany letters, 170 (1) (2023)
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Titre : How much do we know and how much do we care about genetic diversity of threatened plants ? A case study from the French flora Type de document : Imprimé Auteurs : Juliette Ducrettet ; Sandrine Maurice ; Éric Imbert Année de publication : 2023 Article en page(s) : 110-118 Langues : Anglais (eng) Catégories : [CBNPMP-Thèmes] Diversité génétique
[CBNPMP-Thèmes] Statut des espèces & des milieux, degré de menace, extinction, régression, plantes rares, Livre Rouge
[CBNPMP-Thèmes] Conservation et gestion des espèces
[CBNPMP-Géographique] FranceMots-clés : Arenaria grandiflora L. Convolvulus lanuginosus Pinguicula grandiflora Lam. Agropyron cristatum Vitis vinifera L. Delphinium montanum DC. Aster pyrenaeus Desf. ex DC., 1805 Biscutella neustriaca Ranunculus nodiflorus L., 1753 Narcissus triandus Eryngium viviparum J. Gay. Centaurea corymbosa Pourret Brassica insularis Moris Cypripedium calceolus L., 1753 Anchusa crispa Viv. Eryngium alpinum L., 1753 Liparis loeselii (L.) L.C.M. Richard Ranunculus lateriflorus Résumé : mall demographic size and reduced genetic diversity increase the extinction probability of a population. Genetic diversity within populations for neutral markers is an heuristic parameter, as it reflects past and ongoing processes acting on populations, and informs on possible futures. This study presents a synthesis of the knowledge on neutral genetic diversity for threatened plants in metropolitan France and the use of such data in management actions. Pairing threatened species with the closest non-threatened species for which we could find appropriate genetic data, we collected information on genetic diversity for 25 threatened species and non-threatened controls. We found that threatened species in France have on average a lower within-population genetic diversity than non-threatened controls and thus are exposed to risks linked to genetic erosion. Combining an analysis of recovery plans and a survey addressed to practitioners, we concluded that genetic diversity is rarely integrated in management actions. The integration of such information into applied conservation remains a challenge. We provide some guidelines to collect biological traits impacting within-population genetic diversity and some specific recommendations for a better incorporation of population genetics concepts into conservations actions. Lien pérenne : DOI : 10.1080/23818107.2022.2125902 Permalink : https://biblio.cbnpmp.fr/index.php?lvl=notice_display&id=153215
in Botany letters > 170 (1) (2023) . - 110-118Ducrettet, J., Maurice, S., Imbert, É. 2023. How much do we know and how much do we care about genetic diversity of threatened plants ? A case study from the French flora. Botany letters, 170(1): 110-118.Exemplaires (1)
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